786-O Xenograft Model | Xenograft Services (2024)

786-O Xenograft Model | Xenograft Services (1)

786O Cell Line Derived Xenograft

786-O is a human renal cell carcinoma cell line that is often used as a model for the study of kidney cancer. This cell line was derived from a patient with renal adenocarcinoma and has been extensively characterized. The 786-O cell line is commonly used in preclinical research to investigate the biology of kidney cancer, as well as to test new therapeutic approaches. Researchers use this cell line to study the underlying mechanisms of kidney cancer, to evaluate potential drug targets, and to test the efficacy of new drugs in vitro and in animal models.

786-O is a human renal cell carcinoma cell line that was derived from a primary renal tumor in a patient with clear cell carcinoma. It is commonly used as a model for kidney cancer research, as clear cell renal carcinoma is the most common subtype of renal cell carcinoma.

The 786-O cell line has several characteristics that make it a useful tool for cancer research. It is able to grow in culture and exhibits many of the same properties as renal cancer cells found in patients. It is also resistant to many commonly used chemotherapeutic agents, making it useful for testing new cancer treatments.

Researchers have used the 786-O cell line to study the molecular mechanisms of kidney cancer, including the genes and pathways involved in cancer cell growth, invasion, and metastasis. The 786-O cell line has also been used to test the efficacy of various cancer treatments, including chemotherapy, radiation therapy, and immunotherapy.

Overall, the 786-O cell line has contributed significantly to our understanding of kidney cancer biology and has helped in the development of new treatments for this disease.

Human cancer cell lines are commonly injected into immunocompromised mice to grow xenograft tumors. The 786-O cell line derived xenograft (CDX) permits improved targeting of highly vascularized renal cell carcinoma tumors. Sunitinib is a small molecule, receptor tyrosine kinase inhibitor (RTK) with anti-angiogenic activity in humans and xenograft models. The 786-O CDX model lends itself to the testing of another anti-angiogenesis agent, physapubescin, along with the tumor growth inhibitor miR-27a (RNAi).

786-OCDKN2A (mut), p53 (mut), PTEN (mut)
OriginKidney
DiseaseAdenocarcinoma
Metastatic Models (Kidney)N/A
Non-Metastatic Models (Kidney)A498, 786-O, Caki-1, HEK-293, Renca

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786-O Xenograft Model

What is a Xenograft?

Development of an anti-cancer therapeutic requires intense, well planned studies that follow a streamlined path for success. Primary studies are performed in an in vitro setting that allows for high throughput screening and analysis of multiple compounds of interest. This method enables a focused compound screening approach of multiple cell lines within a specific cancer type, or a divergent approach across a broad range of cancer types. Ultimately, in vitro screening results need to be confirmed in an animal model due to in vitro inadequacies of cells cultured on plastic, as this method is far removed from the micro environment of a tumor.

As the logical next step in therapeutic development is the administration of the test compound in a living animal, a cell line derived xenograft model (CDX) is created by inoculating human cancer cell lines in test animals. The injected cell lines grow into established tumors, thus, permitting efficacy studies of the test compounds. An alternative to CDX models is the patient derived tumor xenograft (PDX) which consists of implanting human tumor fragments directly in a mouse model. The PDX model avoids concerns with the CDX model since the tumor is never grown on plastic and there is no selection for single cell populations. In contrary to CDX models, the ideology of PDX models is to maintain the cell population, structure and stroma of the initial tumor.

Why use Xenograft Models?

Cell line derived xenograft (CDX) models or patient derived tumor xenograft (PDX) models enable a larger realm of parameters to be studied not capable with in vitro studies. The complete animal system model expands the scope of studies available to include the effect of test compounds on pharmacokinetics (PK), pharmacodynamics (PD), alternate routes of delivery, inhibition of metastasis, CBCs, dosing regimens, dose levels, etc. However, one of the major drawbacks of CDX and PDX models is that the human cancer cell lines or human patient derived tumors must be implanted in immunocompromised mice in order to bypass the graft versus host rejection by the animal. With the increasing focus of the immune systems role in the recognition and elimination of tumor cells (i.e. immunotherapy), major consideration must be taken into account during experimental concept design of the limitation of checkpoint inhibitors or desired immune response involvement in tumor efficacy. Similarly, any tumor regression after treatment with a test compound in these models will not exhibit the potential complement cascade or innate immune response of the injected therapeutic in humans.

What we offer?

Our in vivo xenograft service department evaluates the efficacy of preclinical and clinical cancer therapeutics utilizing more than 50 validated immunocompromised xenograft mouse models. The value of utilizing our xenograft service department is highlighted by the ability to completely characterize the efficacy, dose regimen, dose levels and optimal combination ratios of lead compounds for cancer, obesity, diabetes, infections and immunology research.

During the design and execution of the xenograft study, our scientists will communicate with and assist the client’s decisions regarding these details:

  • Study Group Formation: classification of mice by body weight, tumor size or other parameters
  • Cancer Cell Line: use of in-house cell lines or utilization of customer-provided cell lines
  • Tumor Implantation: intraperitoneal, subcutaneous, submuscular or intravenous
  • Test Compound Administration: intraperitoneal, intravenous, tail vein, subcutaneous, topical, oral gavage, osmotic pumps or subcutaneous drug pellets
  • Sample Collection: Tumors/tissues can be fixed in 10% NBF, frozen in liquid N2 or stabilized in RNAlater; blood chemistry analysis can be performed throughout the in-life portion of study

Vivarium

Our vivarium is designed such that it enables cost-effective and first-rate preclinical effectiveness testing services. All animal handling and maintenance is regulated following IACUC guidelines. Our facility consists of the following:

  • IACUC-regulated and GLP-compliant
  • Controlled, limited access lab areas
  • Disposable cages
  • Sterile food and water
  • SPF (specific pathogen-free) animals to guarantee pathogens do not interfere with the experiment
  • Established animal handling and micro-injection equipment systems, including an animal health observation program
  • All studies follow pre-approved SOPs

Our staff understands that each proposed study design is unique and customized to the client’s needs. We also recognize the importance of the delivered results as being confidential, highly reproducible and that 100% of the intellectual property (IP) is owned by the client.

In order to receive a quote for your xenograft study, email usthe specific details listed below in order to efficiently begin the study quote process:

  • Cancer cell line(s) used in the study
  • Number (n=) of animals in each study group
  • Number of study groups and control groups
  • Tumor implantation route
  • Administration route of test compound
  • Species of immunocompromised mouse (e.g. NOD/SCID, athymic Nude)
  • Treatment and dose schedule
  • Study endpoint and analysis (e.g. tumor growth delay, PK/PD, survival, toxicity, drug combinations)
  • Samples collected: tumor and tissues to be collected, including storage condition (e.g. snap frozen, RNAlater, 10% NBF, nucleic acid isolation)

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786O Xenograft Model

786-O Xenograft Model | Xenograft Services (2024)

FAQs

What is the doubling time for 786o? ›

Doubling time: 45 hours (PubMed=721102); 24 hours (PubMed=25984343); ~24 hours (CLS=300107); 22.4 hours (NCI-DTP=786-0).

What is a Xenograft model? ›

Xenograft models are based on the implantation of human tumor cells into immunocompromised mice to avoid graft versus host reaction of the mouse against the human tumor tissue. Reaction Biology's in vivo tumor models are derived from a variety of origins such as breast, colon, lung, skin, blood.

What is the xenograft model of breast cancer? ›

Breast cancer xenograft models are used to study the biology of breast cancer, including the mechanisms of tumor growth and metastasis, as well as the tumor's response to various treatments types.

What is xeno graft? ›

(ZEE-noh-graft) The transplant of an organ, tissue, or cells to an individual of another species.

What is the rule of 70 doubling time? ›

The Rule of 70 Formula

Hence, the doubling time is simply 70 divided by the constant annual growth rate. For instance, consider a quantity that grows consistently at 5% annually. According to the Rule of 70, it will take 14 years (70/5) for the quantity to double.

What is the rule of 70 calculator? ›

The rule of 70 calculates the years it takes for an investment to double in value. It is calculated by dividing the number 70 by the investment's growth rate. The calculation is commonly used to compare investments with different annual interest rates.

What are the disadvantages of the xenograft model? ›

One major disadvantage of subcutaneous xenograft tumor models is that the microenvironment of the implanted tumor does not reproduce the environment in which the tumor grows [47][48][49].

What is the most common xenograft used? ›

A xenograft refers to tissue taken from one species and placed into another species. For intraoral bone replacement grafts, the most common animal sources are bovine and porcine.

What are the risks of xenograft? ›

Viral Infections after Xenotransplantation

Viral infections carried by transplanted organs may be activated by immunosuppression, inflammation associated with ischemia–reperfusion injury, graft rejection, or reduced antiviral immune responses in major histocompatibility complex–mismatched grafts.

What is a xenograft procedure? ›

Xenotransplantation is any procedure that involves the transplantation, implantation or infusion into a human recipient of either (a) live cells, tissues, or organs from a nonhuman animal source, or (b) human body fluids, cells, tissues or organs that have had ex vivo contact with live nonhuman animal cells, tissues or ...

What is the purpose of a Xenograft? ›

Cancer drugs and other types of treatment may be tested on xenografts to see how well they work before they are given to the patient. Patient-derived xenografts may be used to help plan treatment and learn what the best treatment may be for a patient. They are also being used in the development of new cancer drugs.

Is xenograft permanent or temporary? ›

Allograft and xenograft skin grafts are usually temporary. They cover the damaged skin until the wound heals or the person grows enough healthy skin to use for a permanent skin graft.

What is an example of a xenograft? ›

The most commonly used xenograft is the EZ Derm®, which is an aldehyde cross-linked porcine dermis that aids in the recovery of partial-thickness skin loss.

What is the cost of xenograft bone graft? ›

Fix Oss (Dental Bone Graft Granules/ Xenograft Bone Mineral/ Bone Graft Material) at Rs 3980/unit in Chennai.

What is allograft vs xenograft model? ›

Allografts are carefully selected and processed human bone materials, while xenografts are derived from animals and possess similar chemical composition to human bone. Synthetic materials such as ceramics and bioactive glasses are used for small defects but may lack osteoinductivity and moldability.

How to calculate doubling time? ›

There is an important relationship between the percent growth rate and its doubling time known as “the rule of 70”: to estimate the doubling time for a steadily growing quantity, simply divide the number 70 by the percentage growth rate.

How do you calculate doubling a number? ›

We can double any number in two ways. 1) Multiply the number by 2. 2) Add the number to itself. Example: Michelle has 4 balls, and Jane has double the number of balls that Michelle has.

What is the doubling time of 661W? ›

Cellosaurus 661W (CVCL_6240) Doubling time: ~24 hours (PubMed=12407171).

How to calculate doubling time of bacteria using OD? ›

Bacteria doubling time is usually calculated by first measuring OD600nm absorbance and then plot the log2(OD600nm) readings against the time. The doubling time represents the time bateria takes to double their amount in cell number when they are within exponential growth phase.

References

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